Automated Bone Marrow Analysis : Dream or Reality?
نویسنده
چکیده
Since 1997, the availability, in haematology laboratories of automated analyzers capable of performing an extended differential blood cell count, including the nucleated red blood cell (NRBC) count, allowed their experimental use to estimate the cell composition of bone marrow fluid. In 1999 we evaluated the Sysmex XE-2100 to detect the clinical usefulness of immature granulocyte (IG) and NRBC counts on more than 300 specimens, using the NCCLS-H20A protocol as reference. This first trial showed for both cell types a predictive negative value > 95 % and a predictive positive value > 90 %. During 2000 we continued evaluation of XE-2100 bone marrow analysis, focusing on:1) a reproducibility study on 85 bone marrow samples assessed by duplicate analysis, which showed CVs of < 4.5 % for cellularity (total nucleated cell count [TNCC]), granulocytes, platelets and RBC parameters (Table 1); 2) a comparison study of XE-2100
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